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dylight 649 conjugated ulex europaeus agglutinin i lectin  (Vector Laboratories)


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    Vector Laboratories dylight 649 conjugated ulex europaeus agglutinin i lectin
    Dylight 649 Conjugated Ulex Europaeus Agglutinin I Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 649 conjugated ulex europaeus agglutinin i lectin/product/Vector Laboratories
    Average 95 stars, based on 55 article reviews
    dylight 649 conjugated ulex europaeus agglutinin i lectin - by Bioz Stars, 2026-03
    95/100 stars

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    CgA + cell numbers are maintained in crypts cultured with M1 or M2 macrophages, while <t>UEA-1</t> + goblet cells and DCAMKL1 + tuft cell numbers are decreased in crypts cultured with M1 macrophages but not M2. A, Representative confocal images showing Chromogranin-A(CgA) expression (white), DAPI (blue) and E- cadherin (red) in crypt-macrophage subtype coculture. B, Histogram showing the average number of CgA positive cells per crypt within each coculture condition ( n = 4, ns). C, Representative confocal images showing UEA-1 expression (green), DAPI (blue) and E-cadherin (red) in each crypt-macrophage subtype coculture. D, Histogram showing the average number of UEA-1 positive cells per crypt within each coculture condition ( n = 5, ** P < .01 *** P < .0001 compared with control). E, Representative confocal images showing DCAMKl1 expression (green), DAPI (blue) and E-cadherin (red) in each crypt-macrophage subtype coculture. F) Histogram showing the average number of DCAMKL1 positive cells per crypt within each coculture condition ( n = 5, * P < .05 compared with control). Scale bar at 20 μm.
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    Image Search Results


    CgA + cell numbers are maintained in crypts cultured with M1 or M2 macrophages, while UEA-1 + goblet cells and DCAMKL1 + tuft cell numbers are decreased in crypts cultured with M1 macrophages but not M2. A, Representative confocal images showing Chromogranin-A(CgA) expression (white), DAPI (blue) and E- cadherin (red) in crypt-macrophage subtype coculture. B, Histogram showing the average number of CgA positive cells per crypt within each coculture condition ( n = 4, ns). C, Representative confocal images showing UEA-1 expression (green), DAPI (blue) and E-cadherin (red) in each crypt-macrophage subtype coculture. D, Histogram showing the average number of UEA-1 positive cells per crypt within each coculture condition ( n = 5, ** P < .01 *** P < .0001 compared with control). E, Representative confocal images showing DCAMKl1 expression (green), DAPI (blue) and E-cadherin (red) in each crypt-macrophage subtype coculture. F) Histogram showing the average number of DCAMKL1 positive cells per crypt within each coculture condition ( n = 5, * P < .05 compared with control). Scale bar at 20 μm.

    Journal: Inflammatory Bowel Diseases

    Article Title: M1 and M2 Macrophages Differentially Regulate Colonic Crypt Renewal

    doi: 10.1093/ibd/izad270

    Figure Lengend Snippet: CgA + cell numbers are maintained in crypts cultured with M1 or M2 macrophages, while UEA-1 + goblet cells and DCAMKL1 + tuft cell numbers are decreased in crypts cultured with M1 macrophages but not M2. A, Representative confocal images showing Chromogranin-A(CgA) expression (white), DAPI (blue) and E- cadherin (red) in crypt-macrophage subtype coculture. B, Histogram showing the average number of CgA positive cells per crypt within each coculture condition ( n = 4, ns). C, Representative confocal images showing UEA-1 expression (green), DAPI (blue) and E-cadherin (red) in each crypt-macrophage subtype coculture. D, Histogram showing the average number of UEA-1 positive cells per crypt within each coculture condition ( n = 5, ** P < .01 *** P < .0001 compared with control). E, Representative confocal images showing DCAMKl1 expression (green), DAPI (blue) and E-cadherin (red) in each crypt-macrophage subtype coculture. F) Histogram showing the average number of DCAMKL1 positive cells per crypt within each coculture condition ( n = 5, * P < .05 compared with control). Scale bar at 20 μm.

    Article Snippet: PE (Phycoerythrin)-conjugated Ulex europaeus lectin (UEA-1) was acquired from VectorLabs to label goblet cells.

    Techniques: Cell Culture, Expressing, Control

    Physical contact between M1 macrophages and colonic crypts but not secretory factors decrease UEA-1+ and DCAMKL1+ cell expression in crypts in vitro. A, Representative confocal images showing UEA-1 expression (green), nuclei (blue) and E-cadherin (red) in crypts cultured in the M1 coculture, M-CM media and M1 only culture models. B, Representative confocal images showing DCAMKL1 expression (green), nuclei (blue) in crypts cultured in the M1 coculture, M1-CM and M1 only culture models. C, (i) Histogram showing the average number of UEA-1 positive cells per crypt cultured in the M1 coculture, M1-CM and M1 only culture models and histograms showing the average number of UEA-1 positive cells per crypt region (C) (ii) base, (iii) mid and (iiv) top when cultured in the M1 coculture, M1-CM and M1 only culture models ( n = 6, * P < .05 compared with control; $$$ P < .001 compared with M1 coculture). Scale bar 20 μm. D, Histogram showing the average number of DCAMKL1 positive cells per crypt cultured in the M1 coculture, M1-CM and M1 only culture models and histograms showing the position of DCAMKL1+ cells per crypt region (ii) base (iii), mid (iv), and top in M1 coculture, M1-CM and M1 only culture models ( n = 6, * P < .05 compared with control). Scale bars 20 μm.

    Journal: Inflammatory Bowel Diseases

    Article Title: M1 and M2 Macrophages Differentially Regulate Colonic Crypt Renewal

    doi: 10.1093/ibd/izad270

    Figure Lengend Snippet: Physical contact between M1 macrophages and colonic crypts but not secretory factors decrease UEA-1+ and DCAMKL1+ cell expression in crypts in vitro. A, Representative confocal images showing UEA-1 expression (green), nuclei (blue) and E-cadherin (red) in crypts cultured in the M1 coculture, M-CM media and M1 only culture models. B, Representative confocal images showing DCAMKL1 expression (green), nuclei (blue) in crypts cultured in the M1 coculture, M1-CM and M1 only culture models. C, (i) Histogram showing the average number of UEA-1 positive cells per crypt cultured in the M1 coculture, M1-CM and M1 only culture models and histograms showing the average number of UEA-1 positive cells per crypt region (C) (ii) base, (iii) mid and (iiv) top when cultured in the M1 coculture, M1-CM and M1 only culture models ( n = 6, * P < .05 compared with control; $$$ P < .001 compared with M1 coculture). Scale bar 20 μm. D, Histogram showing the average number of DCAMKL1 positive cells per crypt cultured in the M1 coculture, M1-CM and M1 only culture models and histograms showing the position of DCAMKL1+ cells per crypt region (ii) base (iii), mid (iv), and top in M1 coculture, M1-CM and M1 only culture models ( n = 6, * P < .05 compared with control). Scale bars 20 μm.

    Article Snippet: PE (Phycoerythrin)-conjugated Ulex europaeus lectin (UEA-1) was acquired from VectorLabs to label goblet cells.

    Techniques: Expressing, In Vitro, Cell Culture, Control